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Table 1 Real-time quantitative RT-PCR probes and primers for turkey cytokines and primers for APEC genes used in this study

From: Analysis of immune responses induced by avian pathogenic Escherichia coli infection in turkeys and their association with resistance to homologous re-challenge

Target

Primer/Probe

Sequence (5′- 3′)

Species

Reference

Cytokines

    

28S

Probe

(FAM)-AGGACCGCTACGGACCTCCACCA-(TAMRA)

C, T

[22]

 

Forward

GGCGAAGCCAGAGGAAACT

  
 

Reverse

GACGACCGATTTGCACGTC

  

IL-1β

Probe

(FAM)-CCACACTGCAGCTGGAGGAAGCC-(TAMRA)

C, T

[22]

 

Forward

GCTCTACATGTCGTGTGTGATGAG

  
 

Reverse

TGTCGATGTCCCGCATGA

  

CXCLi2

Probe

(FAM)-TCTTTACCAGCGTCCTACCTTGCGACA-TAMRA

C,T

[23]

 

Forward

GCCCTCCTCCTGGTTTCAG

  
 

Reverse

TGGCACCGCAGCTCATT

  

IFN-γ

Probe

(FAM)-AAAGATATCATGGACCTGGCCAAGCTTCA-(TAMRA)

T

[23]

 

Forward

AACCTTCCTGATGGCGTGAA

  
 

Reverse

CTTGCGCTGGATTCTCAAGTC

  

IL-13

Probe

(FAM)-TGCCAGCTGAGCACCGACAACG-(TAMRA)

T

[23]

 

Forward

CCTGCACGGCCAGATGA

  
 

Reverse

GGCAAGAAGTTCCGCAGGTA

  

IL-10

Probe

(FAM)-CCTGAAGATGACAATGAAGCGCTGTCA-(TAMRA)

T

[23]

 

Forward

CGACCTGGGCAACATGCT

  
 

Reverse

CCTCTCGCAGGTGAAGAAGTG

  

TGF-β4

Probe

(FAM)-ACCCAAAGGTTATATGGCCAACTTCTGCAT-(TAMRA)

C, T

[23]

 

Forward

AGGATCTGCAGTGGAAGTGGAT

  
 

Reverse

CCCCGGGTTGTGTTGGT

  

APEC genes

    

iss

Forward

ATCACATAGGATTCTGCCG

-

[24]

 

Reverse

CAGCGGAGTATAGATGCCA

  

iuc D

Forward

ACAAAAAGTTCTATCGCTTCC

-

[25]

 

Reverse

CCTGATCCAGATGATGCTC

  

cvi/cva C

Forward

TGGTAGAATGTGCCAGAGCAAG

-

[24]

 

Reverse

GAGCTGTTTGTAGCGAAGCC

  

tsh

Forward

ACTATTCTCTGCAGGAAGTC

-

[24]

 

Reverse

CTTCCGATGTTCTGAACGT

  
  1. C = chicken, T = turkey.