Table 1 Primers used in this study
From: Edwardsiella tarda Hfq: impact on host infection and global protein expression
Primer | Sequences (5′ → 3′)a |
|---|---|
F1 | ATGGCTAAGGGGCAATCT |
R1 | TTATTCAGCGTCATCACTGC |
F2 | GGATCC GGATTGATCCGGTCGCC (BamHI) |
R2 | CTGCCTGCTTGCCCCTTAGCCATTCT |
F3 | GGCAAGCAGGCAGCAGCTACCA |
R3 | GGATCC CGGTCGGTCTCCAACTG (BamHI) |
F4 | GATATC ATGGCTAAGGGGCAATC (EcoRV) |
R4 | GATATC TTCAGCGTCATCACTGC (EcoRV) |
HP1-F | CCCGGG ATGCGAGCTGAAACCATTCTG (SmaI) |
HP1-R | CCCGGG TTTTAAGCATGATGCGCT (SmaI) |
HP2-F | TACGTA ATGCTGATAATGCAAATATCAACT (SnaBI) |
HP2-R | TACGTA CAGGTGCGCGTTAAACCACG (SnaBI) |
DPFP-F | GATATC ATGTCTCAGCCTCAGAGCG (EcoRV) |
DPFP-R | GATATC CAGCGTCAGCAAACGCGT (EcoRV) |