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Figure 4 | Veterinary Research

Figure 4

From: Recombinant adenovirus expressing the haemagglutinin of peste des petits ruminants virus (PPRV) protects goats against challenge with pathogenic virus; a DIVA vaccine for PPR

Figure 4

Effect of virus-vectored ovine IL-2 and/or GMCSF on T-cell responses induced in goats vaccinated with Ad expressing the H protein of PPRV. Goats were vaccinated intra-muscularly with 1 × 109 IU of each Ad virus as described in Table 1 and were challenged intranasally with 1 × 105 TCID50 of pathogenic PPRV, Ivory Coast/89, 15 weeks later. PBMCs were stimulated with H peptides and H-specific T-cell proliferative responses were measured by 3H-thymidine incorporation (A); and H-specific CD8+ IFNγ+(B) and CD4+ IFNγ+(C) cells were analysed by flow cytometry. Results are expressed as the mean stimulation index or mean % IFNγ-containing cells ± S.E. of 3 to 4 goats.

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