Skip to main content
Figure 3 | Veterinary Research

Figure 3

From: Piscine orthoreovirus (PRV) infects Atlantic salmon erythrocytes

Figure 3

PRV-positive RBC detected by flow cytometry. Detection of PRV protein in isolated RBC from Experimental Challenge # 2 by flow cytometry. (A) Density plot showing the gating strategy for intracellular staining. FS, forward scatter; SS, side scatter. (B) Negative control from 0 weeks post challenge (wpc) for intracellular staining, representing the background fluorescence. (C) Flow cytometry result from the intracellular staining at 5, 7 and 8 wpc. 50 000 cells were counted for each sample. Individual F75 was excluded due to technical difficulties. (D) Plots of side scatter (SS) and fluorescence intensity (PRV ơ1) from 0, 5, 7 and 8 wpc. PRV negative (-), low positive (+) and high positive (++) population are indicated at 7 and 8 wpc.

Back to article page