Table 1 Primer sequences, annealing temperatures of primer sets (°C), expected PCR fragment sizes (bp) and accession numbers or references
Primer name | Primer sequence | Annealing temperature (s) (°C) | PCR product (bp) | Accession number or reference |
|---|---|---|---|---|
ActB | CACGCCATCCTGCGTCTGGA | 63 | 100 | [38] |
Beta actin | AGCACCGTGTTGGCGTAGAG | |||
B2MI | CAAGATAGTTAAGTGGGATCGAGAC | 58 | 161 | [38] |
Beta-2-microgobulin | TGGTAACATCAATACGATTTCTGA | |||
CISH | GGGAATCTGGCTGGTATTGG | 62 | 126 | BE014034 |
Cytokine-inducible SH2-containing protein | CCGACAGTGTGAACAGGTAG | |||
GAPDH | CTTCACGACCATGGAGAAGG | 63 | 170 | AF017079 |
Glyceraldehyde-3-phosphate dehydrogenase | CCAAGCAGTTGGTGGTACAG | |||
HMBS-2 | AGGATGGGCAACTCTACCTG | 58 | 83 | [38] |
Hydroxymethylbilane synthase 2 | GATGGTGGCCTGCATAGTCT | |||
HPRT-1 | GGACTTGAATCATGTTTGTG | 60 | 91 | [38] |
Hypoxanthine phosphoribosyltransferase 1 | CAGATGTTTCCAAACTCAAC | |||
IL-1β | AGAAGAGCCCATCGTCCTTG | 62 | 139 | NM_001005149 |
Interleukine 1 beta | GAGAGCCTTCAGCTCATGTG | |||
IL-6 | ATCAGGAGACCTGCTTGATG | 62 | 177 | NM_214399 |
Interleukine 6 | TGGTGGCTTTGTCTGGATTC | |||
IL-8 | TCCTGCTTTCTGCAGCTCTC | 62 | 100 | NM_213867 |
Interleukine 8 | GGGTGGAAAGGTGTGGAATG | |||
IFNα (1-17) | GGCTCTGGTGCATGAGATGC | 64 | 197 | [39] |
Interferon alpha (Type I) | CAGCCAGGATGGAGTCCTCC | |||
IFNβ | ATGTCAGAAGCTCCTGGGACAGTT | 62 | 246 | [39] |
Interferon beta (Type I) | AGGTCATCCATCTGCCCATCAAGT | |||
IFNγ | GCTCTGGGAAACTGAATGAC | 60 | 167 | NM_213948 |
Interferon gamma (Type II) | TCTCTGGCCTTGGAACATAG | |||
IFNλ1/IL-29A | GAGGCTGAGCTAGACTTGAC | 60 | 115 | NM_001142837 |
Interferon lambda1 (Type III) | CCTGAAGTTCGACGTGGATG | |||
IFNλ3/IL-28B | GGCTCCTTGGCGAACTCATC | 62 | 173 | GQ996936 |
Interferon lambda3 (Type III) | TCCTTCTTCTGGGCCTCCTG | |||
iNOS | GAGAGGCAGAGGCTTGAGAC | 62 | 178 | BI344008 |
Inducible nitric oxide synthase | TGGAGGAGCTGATGGAGTAG | |||
Mx1 | AGTGTCGGCTGTTTACCAAG | 60 | 151 | NM_214061 |
Myxovirus resistance 1 | TTCACAAACCCTGGCAACTC | |||
Mx2 | CCGACTTCAGTTCAGGATGG | 62 | 156 | AB258432 |
Myxovirus resistance 2 | ACAGGAGACGGTCCGTTTAC | |||
OAS1 | CCCTGTTCGCGTCTCCAAAG | 64 | 303 | NM_214303 |
2′-5′-Oligoadenylate synthetase 1 | GCGGGCAGGACATCAAACTC | |||
SIV M protein | AGATGAGTCTTCTAACCGAGGTCG | 62 | 100 | [40] |
TGCAAAAACATCTTCAAGTCTCTG | ||||
PKR | GACATCCAAAGCAGCTCTCC | 62 | 365 | NM_214319 |
Protein Kinase RNA-dependent | CGCTCTACCTTCTCGCAATC | |||
RPL-19 | AACTCCCGTCAGCAGATCC | 60 | 147 | [41] |
Ribosomal protein L19 | AGTACCCTTCCGCTTACCG | |||
RIG-I | CGACATTGCTCAGTGCAATC | 60 | 126 | NM_213804 |
Retinoic acid-inducible gene I | TCAGCGTTAGCAGTCAGAAG | |||
SDHA | CTACAAGGGGCAGGTTCTGA | 58 | 141 | [38] |
Succinate dehydrogenase complex subunit A | AAGACAACGAGGTCCAGGAG | |||
SOCS1 | CGCCCTCAGTGTGAAGATGG | 62 | 110 | EW101597 |
Suppressor of cytokine signaling 1 | GCTCGAAGAGGCAGTCGAAG | |||
SOCS3 | CAGCTCCAAGAGCGAGTACC | 61 | 179 | NM_001123196 |
Suppressor of cytokine signaling 3 | TGACGCTGAGCGTGAAGAGG | |||
TBP-1 | AACAGTTCAGTAGTTATGAGCCAGA | 60 | 153 | [38] |
TATA box binding protein 1 | AGATGTTCTCAAACGCTTCG | |||
TLR3 | CCTGCATTCCAGAAGTTGAG | 60 | 152 | NM_001097444 |
Toll Like Receptor 3 | TGAGGTGGAGTATTGCAGAG | |||
TLR7 | TCAGCTACAACCAGCTGAAG | 60 | 140 | NM_001097434 |
Toll Like Receptor 7 | CAGATGTCGCAACTGGAAAG | |||
TLR8 | AGCGCGGGAGGAGTATTGTG | 62 | 118 | NM_214187 |
Toll Like Receptor 8 | GCCAGGGCAGCCAACATAAC | |||
TNFα | CCAATGGCAGAGTGGGTATG | 62 | 116 | X54859 |
Tumor Necrosis Factor alpha | TGAAGAGGACCTGGGAGTAG |