Figure 2From: Amino acid changes in the spike protein of feline coronavirus correlate with systemic spread of virus from the intestine and not with feline infectious peritonitisRepresentative sequencing pyrograms of codon 1058 in tissue RNA samples. A: Pyrogram with a TTG (codon = leucine) sequence at position 1058 from cat 47, small intestine. The peaks represent a sequence of GCTTTG GG. B: Pyrogram with a CTG (codon = leucine) sequence at position 1058 from cat 55, liver. The peaks represent a sequence of GCTCTG GG. C: Pyrogram with an ATG (codon = methionine) sequence at position 1058 from cat 70, liver. The peaks represent a sequence of GCCATG GG. The horizontal axis shows the nucleotide dispensation order. E, enzyme-only control; S, substrate-only control. The initial nucleotide injected (C) is irrelevant to the sequence and provides a baseline.Back to article page