Table 1 Primer and probe sequences used in this study
Name | Use | Sequence (5’- 3’) | Position in FCoV (strain 79-11461) genome | Position in FCoV (strain C1Je2) genome |
|---|---|---|---|---|
P009 | qPCR forward primer | AGCAACTACTGCCACRGGAT | 26655..26674 | |
P010 | qPCR reverse primer | GGAAGGTTCATCTCCCCAGT | 26826..26807 | |
Taqman-P1 | FCoV qPCR fluorescent probe | FAM-AATGGCCACACAGGGA | 26781..26802 | |
CAACGC-BHQ-1 | ||||
F614 | Forward pyrosequencing primer | GCHCARTATTAYAATGGCAT | 23436..23460 | |
AATGG | ||||
R766 | Biotinylated reverse pyrosequencing primer | BIO-AAGYCTRGCYTGYACT | 23588..23568 | |
TGCAT | ||||
S680 | Pyrosequencing primer | ACAGCCTCDTTAATAGGVGG | 23502..23524 | |
ATG | ||||
C4 | Positive control oligonucleotide | GTAAAGCCRTAGGAGATCGA | Primer does not target FCoV sequence | Primer does not target FCoV sequence |
CATGTAGTTACACTGATGAG | ||||
TCGATCTCC |