Figure 1

Quantification of 16S rRNA Lawsonia intracellularis LR187/5/83 in experimentally challenged pigs. 16S rRNA gene copy number per ng of genomic DNA was measured by qPCR as described in Materials and methods. The mean copy number of the 16S rRNA gene was expressed in Log10 for each time point assessed at 3 (n = 3), 7 (n = 4), 14 (n = 4), 21 (n = 4), 28 (n = 4), 35 (n = 2) and 42 (n = 3) days post challenge. For age-matched negative controls, 16S rRNA gene was not detected (data not shown). Mean ± standard error are shown. Two-tailed student t-test is shown for the most significant comparisons with 14 dpc: *, P < 0.02; **, P < 0.006 and ***, P < 0.0009.