Primer | Orientationa | Positionb | Sequence (5′ - 3′)c | TAd | Amplicon size |
---|---|---|---|---|---|
Identification of SNP | |||||
5′-PRRe - intron 1 | F | −677 ~ -654 | CAGGGCAATGCAAAGCTGTGGTAG | 65°C | 1306 bp |
R | +629 ~ +607 | GCGGCAGTAGAACTTTGAAACCA | |||
5′-UTRf - exon 2 | F | −44 ~ -25 | CGGAGCTACTGATTTCAACT | 63°C | 1434 bp |
R | +1390 ~ +1371 | GGAAAGAGGTAAGCTGGGTA | |||
Genotyping g | |||||
+401 | F (universal) | +320 ~ +341 | GGGGCATTCATCAGTCTTCCAG | 56°C | 200 bp |
R (universal) | +519 ~ +500 | AAGGTCAGGGTTAGCATGAA | |||
F (T allele) | +382 ~ +402 | TAATTTTGTGGTGAGAATCT A | 138 bp | ||
R (C allele) | +418 ~ +400 | CAACATCACAGTCTAATG G | 99 bp | ||
+408 | F | +320 ~ +341 | GGGGCATTCATCAGTCTTCCAG | 56°C | 200 bp |
R | +519 ~ +500 | AAGGTCAGGGTTAGCATGAA | |||
+428 | F (universal) | +288 ~ +307 | TACCCTCTGCTCAACTTGCT | 67°C | 232 bp |
R (universal) | +519 ~ +500 | AAGGTCAGGGTTAGCATGAA | |||
F (T allele) | +408 ~ +429 | CTGTGATGTTGGGTAGTGTGT C | 112 bp | ||
R (C allele) | +449 ~ +427 | GGCTAGTCATTGTTTCAATAGG C | 162 bp |