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Table 1 Primers used for the identification of SNP, ARMS-PCR and RFLP

From: Identification and genotyping of feline infectious peritonitis-associated single nucleotide polymorphisms in the feline interferon-γ gene

Primer

Orientationa

Positionb

Sequence (5′ - 3′)c

TAd

Amplicon size

Identification of SNP

     

  5′-PRRe - intron 1

F

−677 ~ -654

CAGGGCAATGCAAAGCTGTGGTAG

65°C

1306 bp

 

R

+629 ~ +607

GCGGCAGTAGAACTTTGAAACCA

  

  5′-UTRf - exon 2

F

−44 ~ -25

CGGAGCTACTGATTTCAACT

63°C

1434 bp

 

R

+1390 ~ +1371

GGAAAGAGGTAAGCTGGGTA

  

Genotyping g

     

  +401

F (universal)

+320 ~ +341

GGGGCATTCATCAGTCTTCCAG

56°C

200 bp

 

R (universal)

+519 ~ +500

AAGGTCAGGGTTAGCATGAA

  
 

F (T allele)

+382 ~ +402

TAATTTTGTGGTGAGAATCT A

 

138 bp

 

R (C allele)

+418 ~ +400

CAACATCACAGTCTAATG G

 

99 bp

  +408

F

+320 ~ +341

GGGGCATTCATCAGTCTTCCAG

56°C

200 bp

 

R

+519 ~ +500

AAGGTCAGGGTTAGCATGAA

  

  +428

F (universal)

+288 ~ +307

TACCCTCTGCTCAACTTGCT

67°C

232 bp

 

R (universal)

+519 ~ +500

AAGGTCAGGGTTAGCATGAA

  
 

F (T allele)

+408 ~ +429

CTGTGATGTTGGGTAGTGTGT C

 

112 bp

 

R (C allele)

+449 ~ +427

GGCTAGTCATTGTTTCAATAGG C

 

162 bp

  1. aF: forward; R: reverse.
  2. bThe nucleotide positions start from the first translation start point (+1).
  3. cThe italicizing indicates the target SNP.
  4. dAnnealing temperature.
  5. e5′-proximal regulatory region.
  6. f5′-untranslated region.
  7. gPCR was done with a heating and cooling rate of 3 and 2 °C, respectively.