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Figure 2 | Veterinary Research

Figure 2

From: Induction of protective immune response against both PPRV and FMDV by a novel recombinant PPRV expressing FMDV VP1

Figure 2

VP1 protein replication and expression of rPPRV/VP1 in Vero cells. (A) Cells infected with rPPRV/VP1, N75/1, or mock-infected were fixed and labeled with anti-N75/1 mouse serum for the presence of PPRV protein (red) or anti-FMDV VP1 (Asia-I type) rabbit serum for the presence of the VP1 protein (green). (B) The N75/1 and rPPRV/VP1 particles were respectively purified by sucrose gradient centrifugation with 60%, 40% and 20% density (140 000 g). Lysates of N75/1- or rPPRV/VP1-infected Vero cells, lysates of FMDV JSL/06-infected Vero cells, N75/1 particles and rPPRV/VP1 particles were respectively probed by SDS-PAGE and western blotting using anti-FMDV VP1 (Asia-I type) rabbit serum and anti-PPRV-N rabbit serum respectively. Mock-infected Vero and BHK-21 cells were used as controls.

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Veterinary Research

ISSN: 1297-9716