Figure 3
From: Neonatal influenza infection causes pathological changes in the mouse brain
Immunohistochemical detection of intraperitoneally infected influenza virus in the brain. Influenza virus was detected in the hippocampus (A), the cerebellum (B, inset shows infected Purkinje cells), the thalamus (C), choroid plexus of fourth ventricle (D), and the periventricular region (E) of infected mouse brain at 5 dpi. No viral antigen was detected in the infected mouse brain stained without primary antibody (F) and the thalamus of SPF mouse brain (G). Double-immunofluorescence staining for influenza virus (red, H-I) and neuronal marker (NeuN, green, H) or astrocyte marker (GFAP, green, I) show either virus-infected neurons or astrocytes. Scale bar for A-D, G = 100 μm; for E-F = 200 μm; for H-I = 25 μm.