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Figure 1 | Veterinary Research

Figure 1

From: Vesicular stomatitis virus replicon expressing the VP2 outer capsid protein of bluetongue virus serotype 8 induces complete protection of sheep against challenge infection

Figure 1

Genome maps and analysis of recombinant protein expression. (A) Genome maps of recombinant VSV. VSV*∆G encodes for nucleoprotein (N), phosphoprotein (P), matrix protein (M), and the large RNA polymerase (L). The VSV glycoprotein (G) has been replaced by the GFP gene (denoted by *). VSV*∆G(VP2) and VSV*∆G(VP5) express either VP2 or VP5 antigen of BTV-8 from the fourth transcription unit while GFP is expressed from an additional transcription unit downstream of VP2. VSV∆G(VP2,VP5) expresses VP2 from the fourth and VP5 from the fifth position. (B) Immunofluorescence analysis of Vero cells 8 hpi with either VSV*∆G(VP2), VSV∆G(VP2,VP5), VSV*∆G(VP5), VSV*∆G(VP5-FLAG), VSV∆G(VP2,VP5-FLAG), or VSV*∆G. In the upper panel, expression of VP2 is indicated by red fluorescence using a monoclonal anti-VP2 antibody (clone 13C10). In the lower panel, expression of VP5 is indicated by red fluorescence using a monoclonal anti-FLAG (clone M2) antibody. Expression of GFP is indicated by green fluorescence. Scale bar represents 19 μm.

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Veterinary Research

ISSN: 1297-9716