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Figure 4 | Veterinary Research

Figure 4

From: Vesicular stomatitis virus replicon expressing the VP2 outer capsid protein of bluetongue virus serotype 8 induces complete protection of sheep against challenge infection

Figure 4

Determination of viral RNA loads in blood of BTV-8 challenged sheep. Blood was collected from the animal groups (n = 6) vaccinated with (A) VSV*ΔG, (B) VSV*ΔG(VP5), (C) VSV*ΔG(VP2,VP5), and (D) VSV*ΔG(VP2) every second day after infection with BTV-8. Total RNA was extracted from whole blood samples and RT-qPCR was performed to determine viral RNA load. The quantification cycles (Cq) for detection of fluorescence signals were expressed as 50-Cq for all 24 animals (numbered #1 to #24).

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