Figure 6

Primary DEF cells respond to infection of DK212. The DEF cells were transfected with either the pCAGGS-MDA5-CARD plasmid or the empty plasmid. After 16 h, the cells were infected at a dose of 5 TCID₅₀ by DK212. Supernatants were collected at several time-points and analyzed for their TCID₅₀ titers. Virus titers are expressed as means ± standard deviation in log₁₀ TCID₅₀/mL. For statistical purposes, a value of 1.5 was assigned if the virus was not detected from the undiluted sample.