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Figure 5 | Veterinary Research

Figure 5

From: Differences in the tissue tropism to chicken oviduct epithelial cells between avian coronavirus IBV strains QX and B1648 are not related to the sialic acid binding properties of their spike proteins

Figure 5

Cryo-sections of indicated parts of the oviduct incubated with soluble S1 proteins of the IBV strains QX (A) and B1648 (B). The nuclei were stained with DAPI (blue), binding of the soluble proteins was detected with a Cy3-labeled secondary antibody (red) at the apical side of the epithelium. To test if binding is sialic acid dependent, each approach was performed either with a pretreatment of 300 mU Neuraminidase (+NA) section or without the pretreatment (−NA). Scale bar = 50 μm.

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Veterinary Research

ISSN: 1297-9716