Table 1 Bacterial strains and plasmids used in this work
Bacterial strains/Plasmids | Characteristic | Source/Reference |
|---|---|---|
Brucella ovis | ||
PA | virulent strain, natural NalR | CITA collection |
BoΔwadA | PA in frame deletion mutant in wadA Δ58-679 | This work |
BoΔwadB | PA in frame deletion mutant in wadB Δ49-195 | This work |
BoΔwadC | PA in frame deletion mutant in wadC Δ17-306 | This work |
BoPA-Gm | challenge strain, PA GmR | UN collection |
Brucella melitensis | ||
Rev 1 | reference vaccine | CITA collection |
E. coli | ||
S17-1 λpir | mating strain with plasmid RP4 inserted into the chromosome | Simon et al. [[19]] |
TOP10 F’ | F−lacI q Tn 10 (TetR) mcrA Δ(mrr-hsdRMS-mcrBC) 80lacZ ΔM15 ΔlacX74 recA1alaD139 endA1 nupG | Invitrogen |
Plasmids | ||
pCR2.1 | cloning vector | Invitrogen |
pJQK | derivated of pJQ200KS+; KmR, GmS | Scupham and Triplett [[20]] |
pYRI-12 | 913-bp of B. abortus parental chromosomal DNA containing the wadA deletion allele, generated by PCR and cloned into pCR2.1 | This work |
pYRI-13 | BamHI-XbaI fragment from pYRI-12 cloned into the corresponding sites of pJQK | This work |
pYRI-1 | 570-bp of B. abortus parental chromosomal DNA containing the wadB deletion allele, generated by PCR and cloned into pCR2.1 | Gil-Ramírez et al. [[7]] |
pYRI-2 | BamHI-XbaI fragment from pYRI-1 cloned into the corresponding sites of pJQK | Gil-Ramírez et al. [[7]] |
pYRI-14 | 934-bp of B. ovis parental chromosomal DNA containing the wadC deletion allele, generated by PCR and cloned into pCR2.1 | This work |
pYRI-15 | BamHI-XbaI fragment from pYRI-14 cloned into the corresponding sites of pJQK | This work |