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Figure 1 | Veterinary Research

Figure 1

From: Deciphering why Salmonella Gallinarum is less invasive in vitro than Salmonella Enteritidis

Figure 1

S. Gallinarum is less invasive in vitro than S. Enteritidis regardless of the cell origin. A. Invasive ability of six S. Enteritidis and six S. Gallinarum strains were compared using gentamicin protection assays performed with LMH cells. Bacteria (MOI 10) were deposited on cells for 0.5 h. Grey bars represent the number of adhered and intracellular bacteria recovered after washings and black bars the number of intracellular bacteria only. White bar represents the number of adhered and intracellular bacteria and hatched bars the number of intracellular bacteria obtained for SG 2210 strain when the cell plate was centrifuged immediately after infection. The results correspond to the mean× μstandard deviation of three independent experiments performed in duplicate and are expressed in log10 CFU mL-1. Similar results were reproduced with other cell lines. B. Invasive ability of S. Enteritidis LA5 and S. Gallinarum 287/91 strains were compared using gentamicin protection assays performed in avian (CLEC213 and DF1 cells) and human cell lines (HT-29 and HeLa cells). Bacteria (MOI 10) were deposited on cells for 0.5 h. Grey bars represent the number of adhered and intracellular bacteria and black bars the number of intracellular bacteria. Results correspond to the mean× μstandard deviation of two independent experiments performed in duplicate and are expressed in log10 CFU mL-1. Similar results were obtained with other S. Gallinarum and S. Enteritidis strains.

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