Phylodynamic reconstruction of O CATHAY topotype foot-and-mouth disease virus epidemics in the Philippines

Table 1 Oligonucleotide primers used for either RT-PCR or cycle sequencing of the VP1 region from the FMDV isolates

Primer designation	Primer sequence (5' to 3')	Start - end
Reverse primers
NK72	GAAGGGCCCAGGGTTGGACTC	3558 – 3578
EUR-2B52R	GACATGTCCTCCTGCATCTGGTTGAT	3624 – 3649
O-1D487gR	TAATGGCACCRAAGTTGAA	3372 – 3390
O-1D628R	GTTGGGTTGGTGGTGTTGT	3181 – 3199
Forward primers
O-1C244F	GCAGCAAAACACATGTCAAACACCTT	2469 – 2494
O-1C272F	TBGCRGGNCTYGCCCAGTACTAC	2497 – 2519
O-1C283F	GCCCAGTACTACACACAGTACAG	2508 – 2530
O-1D296F	ACAACACCACCAACCCAAC	3181 – 3199
O-1C499F	TACGCGTACACCGCGTC	2724 – 2740
O-1C605hF	TGGCCAGTGCCGGTAAGGACTTTGAC	2830 – 2855
O-1C605nF	TGGCTAGTGCTGGCAAAGACTTTGAC	2830 – 2855

Start and end locations have been mapped against the Kaufbeuren/FRG/66 type O FMDV isolate (GenBank accession no. X00871) [22].

ISSN: 1297-9716