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Table 2 Presence (+) or absence (-) of virulence-associated genes in RF122 and Newbould 305

From: Fine-tuned characterization of Staphylococcus aureus Newbould 305, a strain associated with mild and chronic mastitis in bovines

Gene name

Biofilm formation

Function

NB305

RF122

ica operon

Biofilm synthesis ica operon

+

+

bap

Biofilm associated protein

-

-

aap

Accumulation-associated protein (associated to biofilm formation)

+*

-

ypfP, ltaA, ltaS, tag and dlt operons

Teichoic and Lipoteichoic acid synthesis

+

+

cap operon

Capsular polysaccharide biosynthesis

+

+

Secreted toxins

Function

NB305

RF122

lukE

Leukocidin LukE precursor

+

+

lukD

Leukocidin LukD precursor

+

+

lukM

Leukocidin chain lukM precursor

-

+

lukF’-PV

Panton-Valentine leukocidin LukF’-PV chain

-

+

lukF

Leukocidin F subunit

+

+

lukS

leukocidin/hemolysin toxin subunit S

+

+

hla

Alpha-hemolysin precursor

+

+

hlb

Beta-hemolysin precursor

+

+

hlg (subunits A, B, C)

Gamma-hemolysin component

+

+

hld

Delta-hemolysin

+

-

atl

Bifunctional autolysin precursor

+

+

tst

Toxic shock syndrome toxin 1

-

+

eta

Exfoliative A

+

+

etb

Exfoliative B

-

+

sea

Staphylococcal enterotoxin A

+

+

sec bov

Bovine variant of Staphylococcal enterotoxin C

-

+

seg

Staphylococcal enterotoxin G

-

+

sei

Staphylococcal enterotoxin I

-

+

sel

Staphylococcal enterotoxin L

-

+

sen

Staphylococcal enterotoxin N

-

+

seo

Staphylococcal enterotoxin O

-

+

Colonisation factors

Function

NB305

RF122

vwb

Secreted von Willebrand factor-binding protein (Wbp) precursor

+

+

vwb Sbo5

SaPI-encoded variant of Wbp carried by SaPIbov5

+

-

clfA

Clumping factor A

+

+

clfB

Clumping factor B

+

+

fnbA

Fibronectin binding protein A

+

+

fnbB

Fibronectin binding protein B

+

-

stl

Transcriptional Repressor SaPI

+

+

eap

Extracellular adherence protein

+

+

sasG

S. aureus surface protein G

+

-

plsC

1-acyl-sn-glycerol-3-phosphate acyltransferase

+

-

sak

Staphylokinase

-

-

spa

Immunoglobulin G binding protein A precursor

+

+

  1. *Newbould 305_2513 possess a CWA domain and is 67% similar to aap gene of S. epidermidis (GeneBank accession number YP_189945).