Table 2 Primers and probe used in this study
Region | Primers and probe | Position | Sequence | Product length |
|---|---|---|---|---|
ORF 3 | Forward primer1 (HEV.Fa) | 5278-5294 | GTGCCGGCGGTGGTTTC | 81 bp |
Forward primer2 (HEV.Fb) | 5278-5296 | GTGCCGGCGGTGGTTTCTG | ||
Reverse primer (HEV.R) | 5340-5359 | GCGAAGGGGTTGGTTGGATG | ||
Probe (HEV.P) | 5300-5320 | FAM-TGACMGGGTTGATTCTCAGCC-BHQ1 |