Figure 2From: Lack of a type-2 glycosyltransferase in the fish pathogen Flavobacterium psychrophilum determines pleiotropic changes and loss of virulence Growth, extracellular proteolytic activity and colony spreading of F. psychrophilum wild-type and fpgA − strains. (A) Growth curve at 12 °C in NBF medium, monitored by determining OD525. (■) wild-type and (●) fpgA− strains. Extracellular caseinolytic activity in cell-free supernatants was determined with azocasein as described by Secades et al. [38]. (□) wild-type and (○) fpgA− strains. EU, enzyme units. (B) Colony spreading of F. psychrophilum strains grown on 1/6NA for 96 h at 20 °C according to Pérez-Pascual et al. [37]. (a) wild-type; (b) fpgA−; and (c) fpgA+ (complemented strain).Back to article page