Table 1 Primer sequences used for real-time PCR analysis of mRNA expression of target and housekeeping genes
From: The innate immune response of equine bronchial epithelial cells is altered by training
Name | Primer sequences forward/reverse (5′ to 3′) | Product (bp) | Exon | Reference |
|---|---|---|---|---|
PGK1 | CGTCAAAGACCTGATGTCCA | 98 | 1 | In silico designed |
TCTTGGCATGCTCATCAAAC | ||||
PPIA | TGGGGAGAAATTTGATGATGA | 116 | 1 | In silico designed |
TGGCAGTGCAGATGAAGAAC | ||||
RPL0 | CCAAGGTTGAAGCAAAGGAA | 99 | 1 | [13] |
AAAGCTGGCTGAATTGGTTG | ||||
FGF-1 | AGAAGAATGGGAGCTGCAAA | 138 | 1 | In silico designed |
GGACTCCTTCAGGCTCCTCT | ||||
FSP-1 | GCCCTGGATGTGATGGTATC | 93 | 1 | In silico designed |
CAGCAGCTCCTTTAGCTCTGA | ||||
E-cadherin | ATCCGCAGCCTCATATCATC | 125 | 1 | In silico designed |
CCCACCTGGGTCATTGTACT | ||||
Keratin19 | GAAGGAGACCATGCAGAACC | 120 | 1 | In silico designed |
CCCTGCTTCAGGTACCAGTC | ||||
IFN-beta | CCCCGAGGACACAATGAACT | 81 | 1 | In silico designed |
ACCAATGCAGCATCCTCCTT | ||||
TNF-alpha | AAGGACATCATGAGCACTGAA | 80 | 1 | In silico designed |
GGGCCCCCTGCCTTCT | ||||
IL-6 | CCTGGTGATGGCTACTGCTT | 102 | 1 | In silico designed |
TGCTGTTTGGTTTTGTCTGC | ||||
CXCL8 | TTGGCCGTCTTCCTGCTTT | 101 | 2 Intron (959Â bp) | In silico designed |
GGTTTGGAGTGCGTCTTGATG |