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Table 2 Comparison of different cell culture conditions for the in vitro culture of EBEC

From: The innate immune response of equine bronchial epithelial cells is altered by training

Matrix coating medium

Collagen I

Collagen IV

Collagen I + collagen IV

Collagen G

Fibronectin + BSA + collagen I

Fibronectin + BSA + collagen IV

BEGM

(+)

(+)

(+)

(+)

-

-

LHC-8

+(+)

++

+

+

-

-

DMEM/F12 + 2% Ultroser

+(+)

+++

++

+(+)

-

-

DMEM/F12 + 10% FBS

-

-

-

-

-

-

50% BEGM +50% DMEM/F12

+

+

+

+

-

-

50% LHC-8 + 50% DMEM/F12

+

(+)

+

(+)

-

-

  1. No growth - ; limited growth for 10 days only (+) ; growth during 10 days only + ; growth during the entire culture period ++/+++.