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Figure 5 | Veterinary Research

Figure 5

From: Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella

Figure 5

IgA responses of cattle immunized with WT and R90T H7 flagellin. Three groups of calves were immunized twice via the intramuscular route 2 weeks apart with either 60 μg of WT H7 flagellin + 5mg Quil A (n = 6), 60 μg of R90T H7 flagellin + 5mg Quil A (n = 6) or 5mg Quil A alone (n = 3) as described in the Materials and methods. Levels of anti WT-H7 specific IgA were determined by end-point ELISA from serum sampled at the times indicated. Animals were immunized at day 0 and day 14. Plots show the log2 transformed endpoint titre values for each animal sample for each of the three groups. The sampling times were a day before immunization, and days, 8, 22 and 28 post-immunization; the groups have been separated to ease visualization.

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