Figure 5From: Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella IgA responses of cattle immunized with WT and R90T H7 flagellin. Three groups of calves were immunized twice via the intramuscular route 2 weeks apart with either 60 μg of WT H7 flagellin + 5mg Quil A (n = 6), 60 μg of R90T H7 flagellin + 5mg Quil A (n = 6) or 5mg Quil A alone (n = 3) as described in the Materials and methods. Levels of anti WT-H7 specific IgA were determined by end-point ELISA from serum sampled at the times indicated. Animals were immunized at day 0 and day 14. Plots show the log2 transformed endpoint titre values for each animal sample for each of the three groups. The sampling times were a day before immunization, and days, 8, 22 and 28 post-immunization; the groups have been separated to ease visualization.Back to article page