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Table 3 Additional primers used in the study

From: Functional analysis of bovine TLR5 and association with IgA responses of cattle following systemic immunisation with H7 flagella

Gene

Accession no.

Orientation

Oligonucleotide sequence (5’ – 3’)

Toll-like receptor 5 (TLR5)

NM_001040501

F

CGATGCCTATTTGTGCTTCA

  

R

CACCACCCGTCTCTAAGGAA

Interleukin 1B (IL1B)

NM_174093

F

TCCGACGAGTTTCTGTGTGA

  

R

TGTGAGAGGAGGTGGAGAGC

Interleukin/CXCL 8 (IL8/CXCL8)

NM_173925

F

CACATTCCACACCTTTCCAC

  

R

GGCAGACCTCGTTTCCATT

Chromosome alignment

NM_001205506

F

AGCAGTGACCAAGAGCAGGT

Maintaining phosphoprotein 1

 

R

TCATAGCACGACAGCAACAA

  1. F and R denote forward and reverse primers respectively.