Figure 3From: Identification of systemic immune response markers through metabolomic profiling of plasma from calves given an intra-nasally delivered respiratory vaccine Metabolomic profiling of bovine plasma by positive ionization UPLC-MS. (A) Total Ion Chromatogram (TIC) of bovine plasma. Peaks relating to L-phenylalanine, L-tryptophan and glycocholic acid are indicated. The percentage of solvent B (99.9% acetonitrile, 0.1% formic acid) is indicated on right Y axis. (B) BPI chromatograms of plasma from a vaccinated calf at day 49 (two weeks after booster dosage), and (C) BPI chromatogram of plasma from a non-vaccinated calf at day 49. Representative differences between treatment groups are at the peaks indicated with retention times 5.00 and 7.73Â min.Back to article page