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Table 3 Identification of matched immunoreactive proteins of B. abortus that reacted with B. abortus -positive bovine antisera

From: Immunoproteomic identification of immunodominant antigens independent of the time of infection in Brucella abortus 2308-challenged cattle

Spot no.

Gene name

Gene ID a

Protein identification

Protein ID a

Accession no a

Sequence length

Locus tag a

Score

M r

p I

Sequence coverage (%)

Subcellular location c

COG functional category d

         

Experimental

Theorectical b

    

118

BruAb2_0325

3341905

aldehyde dehydrogenase

YP_223118.1

Q579C7

500

BruAb2_0325

249

53744

53435

5.64

29

unknown

G: Carbohydrate transport and metabolism

146

BruAb2_0024

3341776

branched chain amino-acid ABC transporter substrate-binding protein

YP_222837.1

Q57A58

471

BruAb2_0024

438

50740

44322

6.43

25

periplasmic space

E: Amino acid transport and metabolism

151

aspC

3339882

aspartate aminotransferase

YP_222177.1

Q57C18

400

BruAb1_1488

193

43812

43554

5.94

18

unknown

F: Nucleotide transport and metabolism

161

BruAb1_0775

3339474

3-hydroxyisobutyryl-CoA hydrolase

YP_221504.1

Q57DZ1

349

BruAb1_0775

258

38120

37802

4.84

27

unknown

H: Coenzyme transport and metabolism

162

tsf

3340671

elongation factor Ts

YP_221867.1

Q57CX8

305

BruAb1_1167

367

32061

31491

5.03

35

cytoplasm

J: Translation, ribosomal structure and biogenesis

164

mdh

3340925

malate dehydrogenase

YP_222574.1

Q57AX1

320

BruAb1_1903

76

33854

33704

5.24

7

cytoplasm

G: Carbohydrate transport and metabolism

169

tbpA

3340057

thiamine transporter substrate binding subunit

YP_222421.1

Q57BC4

334

BruAb1_1742

310

36843

36752

6.06

19

periplasmic space

H: Coenzyme transport and metabolism

178

BruAb1_1058

3341091

cysteine synthase A

YP_221767.1

Q57D78

342

BruAb1_1058

417

34445

36701

5.94

30

cytoplasm

E: Amino acid transport and metabolism

187

BruAb2_0291

3341871

hypothetical protein BruAb2_0291

YP_223086.1

Q579F9

330

BruAb2_0291

267

35457

35251

5.50

26

unknown

R: General function prediction only

203

rpsB

3340672

30S ribosomal protein S2

YP_221868.1

Q57CX7

256

BruAb1_1168

259

29308

27999

5.88

29

ribosome

J: Translation, ribosomal structure and biogenesis

204

ubiG

3340925

3-demethylubiquinone-9 3-methyltransferase

YP_415219.1

Q2YLN5

248

BAB1_1875

410

27653

27486

5.79

31

cytoplasm

E: Amino acid transport and metabolism

207

dapB

3341712

dihydrodipicolinate reductase

YP_223731.1

Q576R4

268

BruAb2_0991

536

28792

27605

5.92

45

cytoplasm

E: Amino acid transport and metabolism

218

BruAb2_0647

3342272

hypothetical protein BruAb2_0647

YP_223419.1

Q577X6

224

BruAb2_0647

302

24961

24805

4.83

34

unknown

Q: Secondary metabolites biosynthesis, transport, and catabolism

227

BruAb2_0628

3342294

metal-dependent hydrolase

YP_223400.1

Q577Z5

237

BruAb2_0628

511

25223

25124

5.58

51

cytoplasm

R: General function prediction only

228

msrA

3341844

methionine sulfoxide reductase A

YP_223747.1

Q576P8

218

BruAb2_1009

167

24230

24017

5.65

20

cytoplasm

O: Posttranslational modification, protein turnover, chaperones

231

BruAb1_1470

3340810

50S ribosomal protein L25

YP_222213.1

Q57BY2

207

BruAb1_1470

171

22369

22383

5.91

47

ribosome

J: Translation, ribosomal structure and biogenesis

239

BruAb1_0588

3339410

Fe-Mn superoxide dismutase

YP_221327.1

Q57EG8

199

BruAb1_0588

359

22526

22540

5.83

37

unknown

P: Inorganic ion transport and metabolism

240

rocF

3341875

arginase

YP_223125.1

P0A2Y1

306

BruAb2_0333

188

33415

33182

5.63

24

unknown

P: Inorganic ion transport and metabolism

243

gpm

3341713

phosphoglyceromutase

YP_223732.1

Q576R3

206

BruAb2_0992

446

22929

22886

6.16

43

cytoplasm

G: Carbohydrate transport and metabolism

253

secB

3339678

preprotein translocase subunit SecB

YP_222709.1

P0C125

163

BruAb1_2047

343

17924

17878

4.89

46

cytoplasm

O: Posttranslational modification, protein turnover, chaperones

254

ndk

3339959

nucleoside diphosphate kinase

YP_221449.1

Q57E46

140

BruAb1_0713

58

15269

15278

5.27

20

cytoplasm

F: Nucleotide transport and metabolism

257

BruAb2_0845

3341366

hypothetical protein BruAb2_0845

YP_223598.1

Q577E7

177

BruAb2_0845

500

18506

18517

5.02

43

unknown

S: Function unknown

263

ohr

3341640

organic hydroperoxide resistance protein

YP_223139.1

Q579A6

140

BruAb2_0347

8

14337

14232

5.63

13

unknown

R: General function prediction only

  1. aGene ID, protein ID, accession no. and locus tag were retrieved from the NCBInr database.
  2. bTheoretical molecular weight from the UniProtKB database entry.
  3. cSubcellular locations were predicted using PSORTb v. 2.0.4.
  4. dCluster of orthologous groups (COG) protein database generated by comparing microbial genomes from the NCBI COG.
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Veterinary Research

ISSN: 1297-9716