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Table 1 Primers and probes used for qPCR

From: Comparative analysis of cytokine transcript profiles within mediastinal lymph node compartments of pigs after infection with porcine reproductive and respiratory syndrome genotype 1 strains differing in pathogenicity

Gene

Primer Forward

Primer Reverse

Probe

nM

nM

nM

β-Actin

5´-cactcctaacgctgtggatcag-3´

5´-ccacttaactatcttgggcttatcg-3´

5´-[6FAM]-cacgtgcttcacgcggcagc-[TAM]-3´

300

300

50

TNF-α

5´-tggccccttgagcatca-3´

5´-cgggcttatctgaggtttgaga-3´

5´-[6FAM]-ccctctggcccaaggactcagatca-[TAM]-3´

900

600

50

IFN-α

5´-tcagctgcaatgccatctg-3´

5´-agggagagattctcctcatttgtg-3´

5´-[6FAM]-tgacctgcctcagacccacagcc-[TAM]-3´

150

600

50

IFN-γ

5´-gaaaagctgattaaattccggtag-3´

5´-aggttagatcttggtgacagatc-3´

5´-[6FAM]-tctgcagatccagcgcaaagccatcag-[TAM]-3´

300

900

50

SOCS1

5´-ttcttcgccctcagtgtgaa-3´

5´-ggcctggaagtgcacgc-3´

5´-[6FAM]-ttcgggccccacaagcatcc-[TAM]-3´

300

300

50

IL-10

5´-tgagaacagctgcatccacttc-3´

5´-tctggtccttcgtttgaaagaaa-3´

5´-[6FAM]-caaccagcctgccccacatgc-[TAM]-3´

300

300

150

TGF-β

5´-agggctaccatgccaattt-3´

5´-ccgggttgtgctggttgt-3´

5´-[6FAM]-cactcagtacagcaaggtcctggctctgta-[TAM]-3´

600

600

50

IL-23p19

5´-agaagagggagatgatgagac-3´

5´-agcaggactgactgccgtcc-3´

5´-[6FAM]-ctgaggatcacagccatccccgc-[TAM]-3´

900

300

50