Primer name | Sequence (5′- 3′) | Primer use |
---|---|---|
pBlue linear Fwd 1 | GGGGATCCACTAGTTCTAGAGCG | Linearization of plasmid |
pBlue linear Rev1 | CGGGCTGCAGGAATTCGATATCAAG | Linearization of plasmid |
HpGGT-flank_fusion1F | CTTGATATCGAATTCCTGCAGCCCGTAACCGGTAAAATCAACACGGACGC | Amplification H. pylori ggt and partial up- and downstream flanking genes |
HpGGT-flank_fusion1R | CGCTCTAGAACTAGTGGATCCCCGCGCTCTTATAAAAAGAAGCCGC | Amplification H. pylori ggt and partial up- and downstream flanking genes |
pBluelinear_Hpggtflank1F | CCAAGGAAAGAATTTTAATCCTATTTAG | Linearization of the recombinant plasmid |
pBluelinear_Hpggtflank1R | CTGTTTTCCTTTCAATCAACAATAATC | Linearization of the recombinant plasmid |
Hpkana_fusion_1F | ATTATTGTTGATTGAAAGGAAAACAGATGATTGAACAAGATGGATTGC | Amplification kanamycin resistance gene |
Hpkana_fusion_1R | CTAAATAGGATTAAAATTCTTTCCTTGGTCAGAAGAACTCGTCAAGAAG | Amplification kanamycin resistance gene |
T7 prom3 | TAATACGACTCACTATAGGG | Sequencing |
M13R | CAGGAAACAGCTATGAC | Sequencing |