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Table 1 Primers used for construction of the H. pylori ggt isogenic mutant strains

From: Role of γ-glutamyltranspeptidase in the pathogenesis of Helicobacter suis and Helicobacter pylori infections

Primer name

Sequence (5′- 3′)

Primer use

pBlue linear Fwd 1

GGGGATCCACTAGTTCTAGAGCG

Linearization of plasmid

pBlue linear Rev1

CGGGCTGCAGGAATTCGATATCAAG

Linearization of plasmid

HpGGT-flank_fusion1F

CTTGATATCGAATTCCTGCAGCCCGTAACCGGTAAAATCAACACGGACGC

Amplification H. pylori ggt and partial up- and downstream flanking genes

HpGGT-flank_fusion1R

CGCTCTAGAACTAGTGGATCCCCGCGCTCTTATAAAAAGAAGCCGC

Amplification H. pylori ggt and partial up- and downstream flanking genes

pBluelinear_Hpggtflank1F

CCAAGGAAAGAATTTTAATCCTATTTAG

Linearization of the recombinant plasmid

pBluelinear_Hpggtflank1R

CTGTTTTCCTTTCAATCAACAATAATC

Linearization of the recombinant plasmid

Hpkana_fusion_1F

ATTATTGTTGATTGAAAGGAAAACAGATGATTGAACAAGATGGATTGC

Amplification kanamycin resistance gene

Hpkana_fusion_1R

CTAAATAGGATTAAAATTCTTTCCTTGGTCAGAAGAACTCGTCAAGAAG

Amplification kanamycin resistance gene

T7 prom3

TAATACGACTCACTATAGGG

Sequencing

M13R

CAGGAAACAGCTATGAC

Sequencing