Figure 6

Intrinsic biological effects of FhTLM are lifestage specific. (A) Effect of rFhTLM on the viability of adult liver flukes. Viability was determined by MTT reduction after of 24 hours of incubation. Results are plotted as mean ± SEM of triplicate readings. from one of two independent experiments. (B) Effects of rFhTLM on the motility and viability of NEJs. Viability of the NEJs was determined by MTT reduction at 24 h of incubation 250 ng/mL of rFhTLM and TGF-β, or PBS. Results are plotted as the mean OD reading values ± SEM from three replicates from one of two independent experiments. Motility was recorded in the same experiments is indicated underneath using a semi-quantitative scale. (C) Biological effect of rFhTLM on egg embryonation. Unembryonated eggs were incubated with rFhTLM and TGF-β at the indicated dose, or dH2O for 7 days and the egg development was examined microscopically. Results are represented as mean ± SEM of % embryonated egg. Data in panel B is analysed by 1-way anova and data in panel C is analysed using 2-way anova. Significant differences are *P < 0.05, **P < 0.01 and ***P < 0.001. All results are representative of one of two independent experiments.