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Figure 3 | Veterinary Research

Figure 3

From: Glutamine starvation enhances PCV2 replication via the phosphorylation of p38 MAPK, as promoted by reducing glutathione levels

Figure 3

Glutamine supplementation blocks the promotion of PCV2 replication by glutamine starvation. PK-15 cells were infected with PCV2 at an MOI of 1 in complete medium for 24 h. These PK-15 cells were subsequently grown in medium containing various concentrations of glutamine for 48 h before the media of all treatment groups were changed to fresh media with 4 mM glutamine. The cells were incubated for another 48 h and then harvested to determine the number of PCV2 log 10 DNA copies per 106 cells (A) and the relative proportion of PCV2 infected cells (B and C). Groups were compared by a 1-way ANOVA followed by a least-significant difference test. Significant changes are indicated by *(P < 0.05) in comparison with control.

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