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Figure 7 | Veterinary Research

Figure 7

From: Glutamine starvation enhances PCV2 replication via the phosphorylation of p38 MAPK, as promoted by reducing glutathione levels

Figure 7

Knockdown of p38 MAPK decreases PCV2 replication but does not affect the level of GSH and MDA in PK-15 cells. PCV2-infected cells were transfected with p38-specific or control siRNA. After 5 h of transfection treatment, the medium was removed, and fresh basal medium was added. After transfected-cells were cultured for another 72 h, the relative mRNA levels of p38 (A), the relative proteins levels of p38 and p-p38 (B and C), the number of PCV2 log 10 DNA copies per 106 cells (D), the relative proportion of infected cells (E), the level of GSH (F) and MDA (G) were assayed as described in Methods. Values are given as mean ± SD from three independent experiments. Groups were compared by a 1-way ANOVA followed by least-significant difference test. Significant changes are indicated by **(P < 0.01) and ## (P < 0.01) in comparison with controls.

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