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Figure 8 | Veterinary Research

Figure 8

From: Glutamine starvation enhances PCV2 replication via the phosphorylation of p38 MAPK, as promoted by reducing glutathione levels

Figure 8

PCV2 replication promoted by glutamine starvation could be blocked by p38 knockdown in PK-15 cells. PCV2-infected cells were transfected with p38-specific, control or non siRNA. After 5 h of transfection treatment, the medium was removed, and fresh basal medium with various concentrations of glutamine was added. After transfected-cells were cultured for another 72 h, the number of PCV2 log 10 DNA copies per 106 cells (A) and the relative proportion of infected cells (B) were assayed by real-time PCR and immunofluorescence microscopy, respectively. Values are given as mean ± SD from three independent experiments. Groups were compared by a 1-way ANOVA followed by least-significant difference test. Significant changes are indicated by **(P < 0.01) and # (P < 0.05) in comparison with controls.

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