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Figure 5 | Veterinary Research

Figure 5

From: The immunoglobulin M-degrading enzyme of Streptococcus suis, Ide Ssuis , is involved in complement evasion

Figure 5

Ide Ssuis promotes survival in opsonophagocytosis assays including purified porcine neutrophilic granulocytes and serum with specific IgM titers. (A) Survival of strain 10 (wt), 10Δide Ssuis (Δ), 10Δide Ssuis _homologue (Δ_h) and 10Δide Ssuis _C-terminus (Δ_C) in opsonophagocytosis assays with serum from a piglet with specific IgM. (B) Attenuation of 10Δide Ssuis in opsonophagocytosis assays depends on active complement and adaptive immunity. The ratios of the survival factors of 10Δide Ssuis to the respective survival factors of wt are shown. A ratio of 1 is depicted by the horizontal line and refers to a lack of attenuation. To access the impact of complement, the post immune serum with specific IgM used for experiments shown in (A) was heat-inactivated or treated with zymosan. Samples with active and heat-inactivated serum from a colostrum-deprived pig were included to investigate whether this Ide Ssuis -mediated phenotype depends on adaptive immunity. Bars and error bars represent mean values and standard deviations, respectively. Significant differences between strains and ratios of survival factors in (A) and (B), respectively, are indicated (* p < 0.05, ** p < 0.01).

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