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Table 1 Comparison of the gene sequences of Stx1 and Stx2 before and after mutagenesis

From: Evaluation of biological safety in vitro and immunogenicity in vivo of recombinant Escherichia coli Shiga toxoids as candidate vaccines in cattle

Gene

Relevant nucleotide sequence (codon triplets for amino acids 164 to 174 in 5′ to 3′ direction)*

Compared to wild type amino acid replacements on position †

stx1 WT

-gtg aca gct gaa gct tta cgt ttt cgg caa ata-

none

stx1 mut

-gtg aca gct Caa gct tta cTt ttt cgg caa ata-

E167Q, R170L

stx2 WT

-gtc aca gca gaa gcc tta cgc ttc agg cag ata-

none

stx2 mut

-gtc aca gca Caa gcc tta cTc ttc agg cag ata-

E167Q, R170L

  1. *Bold letters indicate positions of replaced nucleotides, with small letters marking the nucleotides in wild type toxin sequences and capital letters marking replaced nucleotides in mutant toxin sequences.
  2. †E = glutamine acid, Q = glutamine; R = arginine; L = leucine.
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Veterinary Research

ISSN: 1297-9716