Figure 2

Kinetics of Ki-67 ⁺ CD8β ⁺ and Ki-67 ⁺ CD4 ⁺ T cells in peripheral blood of FLUAVsw-infected pigs. Freshly isolated PBMCs were stained and analyzed for CD4, CD8α, CD8β, CD27, Ki-67 and perforin expression by FCM. (A) CD3⁺CD8β⁺ T cells were gated for Ki-67 expression and Ki-67⁺ cells were further analyzed for expression of CD27 and perforin. Perforin⁺ (red gate) and perforin⁻ (grey gate) Ki-67⁺CD3⁺CD8β⁺ T cells were further subgated for quantitative analysis over the time course following infection. (C) CD3⁺CD4⁺ T cells were gated for Ki-67 expression and Ki-67⁺ cells were further analyzed for CD8α and CD27 expression. CD8α⁺ (red gate) and CD8α⁻ (grey gate) Ki-67⁺CD3⁺CD4⁺ T cells were subgated for quantitative analysis over the time course following infection. (A and C) Exemplary raw data of animals #6 and #8, prior to infection and one week post infection, is shown. (B and D) Absolute numbers of Ki-67-expressing subsets within CD8β⁺ T cells (B) and CD4⁺ T cells (D) of six infected animals in the time course following infection. For calculation of absolute numbers, total lymphocyte counts were multiplied by percent values obtained by gating on the respective T-cell subpopulation.