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Figure 3 | Veterinary Research

Figure 3

From: Magnitude and kinetics of multifunctional CD4+ and CD8β+ T cells in pigs infected with swine influenza A virus

Figure 3

Kinetics of FLUAVsw-specific IFN-γ-producing T cells identified by ELISpot and H1N2-neutralizing antibody titers. For IFN-γ ELISpot, freshly isolated PBMCs were in vitro restimulated for 24 h with FLUAVsw (infection strain; MOI = 0.1). Mock- and medium-incubated cultures served as negative controls. Frequency of IFN-γ-producing cells within 3 × 105 PBMCs is displayed for FLUAVsw- (red line), mock- (dark grey line) and medium-incubated (light grey line) cultures. Neutralizing antibody titers are displayed in dashed grey lines according to the secondary y-axis (for details see caption of Figure 1B). Data of PBS-treated (#1 to #3) and FLUAVsw infected (#4 to #9) animals is shown in the time course following infections.

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