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Figure 1 | Veterinary Research

Figure 1

From: PRRSV-infected monocyte-derived dendritic cells express high levels of SLA-DR and CD80/86 but do not stimulate PRRSV-naïve regulatory T cells to proliferate

Figure 1

Phenotype of porcine moDCs. Four-color FCM including a Live/Dead discrimination dye was used to study CD1, CD14, CD163, CD169, SLA-DR and CD80/86 expression on living CD172ahigh-defined moDCs at days 0, 4 and 7 following in vitro cultivation. For each time point, live CD172ahigh cells were gated and light scatter properties analyzed. Offset histograms show fluorescence intensities of CD1, CD14, CD163 CD169, SLA-DR and CD80/86 and their corresponding isotype controls (con.) within CD172ahigh cells for each time point. Representative data of pig number 5 is shown. Scatter diagrams on the right show MFI (y-axis) for each analyzed surface marker and the corresponding isotype control for experiments with moDCs derived from six different pigs (indicated by different symbols). Coloured bars indicate mean values (right panel).

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