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Figure 3 | Veterinary Research

Figure 3

From: Eimeria bovis infection modulates endothelial host cell cholesterol metabolism for successful replication

Figure 3

Lipid droplet distribution in developing meronts. Bovine umbilical vein endothelial cells (BUVEC) were infected with E. bovis sporozoites. For the illustration of lipid droplets, the cells (17 days pi) were either stained with Bodipy 493/503 and analyzed by conventional fluorescence microscopy (A + C: Bodipy 493/503 (green), B + D: respective phase contrast illustrations) or confocal microscopy (E: Bodipy 493/503 (green), DNA staining via DAPI (blue); illustration in (E) was projected from 25 slices of Z-stack confocal analysis which is given in Additional file 1) or subjected to osmium tetroxide staining (D including close-up). Note massive abundance of lipid droplets (green (Bodipy staining) or black (osmium tetroxide) dots) within immature macromeronts (A, C, F) and the large sizes of lipid droplets (= ld, E). Note also that the cytoplasm of infected host cells is free of lipid droplets (A, C). Scale bars represent 20 μm. nuc = nucleus of the host cell, cyt = cytoplasm of the host cell, PV = parasitophorous vacuole, mac = macromeront-derived plasma membrane, imm = immature macromeront, mat = mature macromeront, ld = lipid droplet.

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