Figure 5From: Eimeria bovis infection modulates endothelial host cell cholesterol metabolism for successful replication E. bovis macromeront formation and merozoiteI production after cholesterol and desmosterol supplementation. E. bovis-infected BUVEC (n = 5) were cultivated in non-supplemented (=controls) or cholesterol- and desmosterol-enriched medium. The effects on meront I formation (A) were assessed microscopically by estimating the rate of meront I-carrying host cells (black bars: cholesterol-enriched medium, grey bars: desmosterol-enriched medium, white bars: controls) and by measuring the sizes of developing meronts I (triangles: cholesterol-enriched medium, circles: desmosterol-enriched medium, squares: controls). The effects on total merozoite Iproduction (B) were quantified via an EbMIC4-based qPCR after 30 days pi.Back to article page