Table 1 Sequences of primers and probes used in real-time qPCR
Symbol | Name | Accession number | PCR efficiency | Amplicon length | Forward (5’→3’) | Reverse (5’→3’) | Probe (reporter 5’-3’ quencher) |
|---|---|---|---|---|---|---|---|
ACAT1 | Acetyl-CoA acetyltransferase 1 | NM_001046075.1 | 0.95 | 102 | TCATATGGGCAACTGTGCTGA | CTGCTTTACTTCTGGTATAG | FAM-AGCATAAGTATCCTGTTCCTCTCGTG-BHQ1 |
ACAT2 | Acetyl-CoA acetyltransferase 2 | NM_001075549.1 | 1.08 | 194 | AGCAGTGGTTCTTATGAA | AGGCTTCATTGATTTCAAA | FAM-ATCAACATCCTCCAGCGACCA-BHQ1 |
HMGCS1 | 3-hydroxy-3-methylglutaryl-CoA synthase 1 | NM_001206578 | 0.93 | 197 | CTACCTCAGTGCATTAGA | CTCTGTTCTGGTCATTAAG | HEX-AAGTCATTCAGCAACATCCGAGC-BHQ1 |
HMGCR | 3-hydroxy-3-methylglutaryl-CoA reductase | NM_001105613.1 | 0.94 | 109 | GCCATCAACTGGATAGAG | CCTCAATCATAGCCTCTG | FAM-TCTCTGACAACCTTGGCTGGAAT-BHQ1 |
SQLE | Squalene epoxidase | NM_001098061.1 | 0.93 | 132 | CCCTTCTTCACCAGTAAA | CCCTTCAGCAATTTTCTC | HEX-AACAACAGTCATTCCTCCACCAGTA-BHQ1 |
CH25H | Cholesterol 25-hydroxylase | NM_001075243.1 | 1.00 | 87 | TTGGGTGTCTTTGACATG | CAGCCAGATGTTGACAAC | FAM-CGTCTTGCTGCTCCAGTGTC-BHQ1 |
SOAT1 | Sterol O-acyltransferase 1 (SOAT1) | NM_001034206.2 | 0.93 | 99 | GCCCTCCTCATTCTCTTC | CCCAAAAGCATAAGACATGAG | FAM-AGCACCAGCCTTCCTTCATCA-BHQ1 |
LDLR | Low density lipoprotein receptor | NM_001166530.1 | 1.00 | 96 | CGCCTACCTCTTCTTTAC | ACCACGTTCTTAAGGTTG | FAM-TCGCTTCGGTCCAGAGTCATC-BHQ1 |
OLR1 | Oxidized low density lipoprotein/(lectin-like) receptor 1 | NM_174132.2 | 0.97 | 102 | CAAGCTGGATGAGAAATC | GGACAAGGACCTGAATAG | TET-ACTTCACCGCCAGAACCTGA-BHQ1 |
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | AF022183 | 0.98 | 82 | GCGACACTCACTCTTCTACCTTCGA | TCGTACCAGGAAATGAGCTTGAC | FAM-CTGGCATTGCCCTCAACGACCACTT–BHQ1 |