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Table 2 BAGV detection by real time RT-PCR in organs from inoculated red-legged partridges

From: Bagaza virus is pathogenic and transmitted by direct contact in experimentally infected partridges, but is not infectious in house sparrows and adult mice

Partridge

Sex

dpi

Viral load in organs (Ct)

Brain

Heart

Kidney

Spleen

Liver

N 1

female

4

29

19

21

21

21

N 4

male

4

30

25

23

23

22

4*

female

6

27

23

23

23

24

N 2

female

7

22

22

22

24

23

N 5

male

7

27

23

25

27

30

10*

male

7

24

21

25

24

25

N 3

female

10

30

29

27

32

31

N 6

male

10

26

28

23

29

33

7*

male

10

21

26

25

28

33

1

male

15

34

>40

37

34

>40

2

female

15

31

32

31

29

37

3

female

15

33

32

32

32

37

5

female

15

31

32

32

31

34

6

male

15

33

35

34

30

38

8

male

15

31

32

30

30

38

9

male

15

35

35

34

31

35

  1. Individual partridges are numbered correlatively (N indicates partridges in the programmed necropsy group). The table indicates the sex of the sampled partridges, the day post infection (dpi) when the samples were obtained and the corresponding viral load expressed in Ct (threshold cycle) obtained by real-time RT-PCR analysis, for the different organs examined.
  2. *Lethally infected birds; Complete sequence analyzed.