Table 2 Study 3: Mortality, mean death time (MDT), and OP virus shedding in 2 and 4 week-old chickens inoculated with m NDV and HPAIV

2 weeks old

HPAIV

6/10 (1.6)

nd

nd

nd

nd

5/7 (7.9 ± 3)^A

3/4 (1.9 ± 0.4)^A

0/4^A

0/4^A

mNDV

-

9/10 (3.7 ± 0.3)^A

9/10 (3.7 ± 0.1)^A

10/10 (4.3 ± 0.3)^A

10/10 (4.8 ± 0.2)^A

nd

nd

nd

nd

mNDV + HPAIV

8/10 (2)

4/4 (3.3 ± 0.6)^A

2/2 (4.1 ± 0.1)^A

2/2 (4.5 ± 0.4)^A

2/2 (3.8 ± 0.2)^A

3/4 (7.3 ± 2.9)^A

0/2^A

0/2^A

0/2^A

mNDV + HPAIV 3 days later

2/10 (7.0)

10/10 (3.4 ± 0.3)^A

9/10 (3.6 ± 0.3)^A

9/9 (3.6 ± 0.3)^A

9/9 (4.8 ± 0.2)^A

0/10**^B

0/10*^A

1/9 (4.5)^A

1/9 (9.3)^A

4 weeks old

HPAIV

8/10 (3.5)

nd

nd

nd

nd

8/9 (7.1 ± 0.9)^A

0/5^A

4/4 (3.3 ± 0.8)^A

0/2^A

mNDV

-

10/10 (3.5 ± 0.2)^A

8/8 (3.5 ± 0.2)^A

8/8 (3.8 ± 0.3)^A

8/8 (4.7 ± 0.2)^A

nd

nd

nd

nd

mNDV + HPAIV

8/10 (2.1)

3/5 (2.7 ± 0.5)^A

2/2 (3 ± 0.9)^A

2/2 (2.9 ± 0.7)^A

2/2 (3.6 ± 0.8)^A

3/5 (6.6 ± 1.7)^A

0/2^A

0/2^A

0/2^A

mNDV + HPAIV 3 days later

1/10 (9.0)

9/10 (3.9 ± 0.3)^A

8/10 (3.4 ± 0.4)^A

9/10 (3.5 ± 0.3)^A

10/10 (4.5 ± 2.1)^A

0/10***^B

0/10 ^A

0/10***^A

1/10 (10)^A