Phenotypic and functional analysis of monocyte populations in cattle peripheral blood identifies a subset with high endocytic and allogeneic T-cell stimulatory capacity

Table 1 Antibodies list

Antigen	Antibody nomenclature	Isotype	Conjugate	Source
CD1b	CC14	IgG1	Nil	IAH, AbD Serotec
CD80	IL-A159	IgG1	Nil	ILRI, AbD Serotec
CD86	IL-A190	IgG1	Nil	ILRI, AbD Serotec
CD40	IL-A156	IgG1	Nil	ILRI, AbD Serotec
MHCII-DR	CC108	IgG1	Nil	IAH, AbD Serotec
CD11b	CC94	IgG1	Nil	IAH
CD11c	NAM4	IgG1	Nil	[44]
CD2	CC42	IgG1	Nil	IAH, AbD Serotec
CD3	MM1A	IgG1	Nil	VMRD, Inc., Pullman, WA
CD4	CC30	IgG2a	R:PE	IAH, AbD Serotec
CD8α	CC63	IgG2a	R:PE	IAH, AbD Serotec
CD8β	CC58	IgG1	R:PE	IAH, AbD Serotec
CD21	CC21	IgG1	Nil	IAH, AbD Serotec
NKp46	GR13.1(EC1.1)	IgG1	Nil	Timothy Connelly (UoE), AbD Serotec
CD26	CC69	IgG1	Nil	IAH
CD172a	CC149	IgG1	Nil	IAH
CD206	3.29B1.10	IgG1	R:PE	Beckman Coulter
TLR2	HuCaL AbD 16476.1		Nil	AbD Serotec
CD163	EDHu-1	IgG1	Nil	AbD Serotec
CD16	KD1	IgG2a	FITC	AbD Serotec
CD14	TUK4	IgG2a	Alexa Fluor 647	AbD Serotec
IgG1 isotype control	AV20	IgG1	Nil	IAH, [35]
IgG2a isotype control	AV29	IgG2a	Nil	IAH, [35]

Monoclonal antibodies used for flow cytometric analysis. IAH, Institute for Animal Health; ILRI, International Livestock Research Institute; UoE, University of Edinburgh.

ISSN: 1297-9716