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Figure 4 | Veterinary Research

Figure 4

From: Identification and characterisation of microsatellite DNA markers in order to recognise the WSSV susceptible populations of marine giant black tiger shrimp, Penaeus monodon

Figure 4

Quantitative assay of WSSV after experimental virus challenge. The amplification plots (A and D) of white spot syndrome virus (WSSV) in WSSV resistant [R1 = samples devoid of 442 bp band and R2 = samples devoid of 236 bp band] and susceptible populations [S1 = samples containing 442 bp band and S2 = samples containing 236 bp band] of Penaeus monodon based on 442 bp and 236 bp microsatellite DNA marker respectively. Genomic DNA from tail tissue was amplified using WSSV (A and D) primers by real-time PCR after 72 h challenge experiment. The mean relative WSSV was expressed as cycle threshold value, CT between [R1 and R2] and [S1 and S2] populations of P. monodon. A two sample t-test showed highly statistically significant difference between these two populations (C and F, n = 240, P < 0.001) using both the microsatellite DNA marker. The dissociation curves for WSSV amplicon are shown in panels (B) and (E) for two populations ([R1 and R2] and [S1 and S2]) differentiated by 442 bp and 236 bp microsatellite DNA marker. The Tm value indicated the same WSSV amplicon in every case.

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