Figure 4From: Identification and characterisation of microsatellite DNA markers in order to recognise the WSSV susceptible populations of marine giant black tiger shrimp, Penaeus monodon Quantitative assay of WSSV after experimental virus challenge. The amplification plots (A and D) of white spot syndrome virus (WSSV) in WSSV resistant [R1 = samples devoid of 442 bp band and R2 = samples devoid of 236 bp band] and susceptible populations [S1 = samples containing 442 bp band and S2 = samples containing 236 bp band] of Penaeus monodon based on 442 bp and 236 bp microsatellite DNA marker respectively. Genomic DNA from tail tissue was amplified using WSSV (A and D) primers by real-time PCR after 72 h challenge experiment. The mean relative WSSV was expressed as cycle threshold value, CT between [R1 and R2] and [S1 and S2] populations of P. monodon. A two sample t-test showed highly statistically significant difference between these two populations (C and F, n = 240, P < 0.001) using both the microsatellite DNA marker. The dissociation curves for WSSV amplicon are shown in panels (B) and (E) for two populations ([R1 and R2] and [S1 and S2]) differentiated by 442 bp and 236 bp microsatellite DNA marker. The Tm value indicated the same WSSV amplicon in every case.Back to article page