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Table 1 Summary of pig groups and corresponding numbers, inoculum and pig diarrhea outcomes after inoculation

From: Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain

Pig Group

Pig numbers

Inoculum dilution a

Calculated inoculum infectious titers (log 10 PFU/mL) b

Calculated inoculum RNA titers (log 10 GE/mL) b

Diarrhea (percent) c

Fecal virus RNA shedding (log 10 GE/mL)

G1

4

10āˆ’3

4

10

4/4 (100)

11.8-13.0

G2

5

10āˆ’4

3

9

5/5 (100)

12.5-12.9

G3

5

10āˆ’5

2

8

5/5 (100)

12.0-13.4

G4

13

10āˆ’6

1

7

13/13 (100)

9.8-13.3

G5

8

10āˆ’7

0

6

8/8 (100)

11.5-13.7

G6

5

10āˆ’8

āˆ’1

5

2/5 (40)

11.4-12.4

G7

4

10āˆ’9

āˆ’2

4

0/4 (0)

-d

G8

5

10āˆ’10

āˆ’3

3

0/5 (0)

-

Control 1g

2

PBS

-

-

0/2 (0)

- or 5.0

Control 2

2

PBS

-

-

0/2 (0)

-

One litter of PEDV-naĆÆve Sow E

12

10āˆ’5(100 PDD50)

2

8

12/12 (100)

11.8-13.0

One litter of PEDV-preexposed Sow F

13

10āˆ’3 (10,000 PDD50)

4

10

0/13 (0)

5.1-8.9 e

One litter of PEDV-preexposed Sow G

10

10āˆ’4 (1,000 PDD50)

3

9

0/10 (0)

4.9-5.5 f

  1. aEach pig was inoculated orally with 3Ā mL inoculum.
  2. bTiters were calculated based on the titer of the original virus pool and dilution times.
  3. cFecal scores of 3 as determined by 24 hpi for pigs in G1-G8 and control groups and conventional pigs of sow E, and through 7 and 9 dpi for conventional pigs of sows F and G, respectively.
  4. dNegative or below detection limit of RT-qPCR (4.8 log10GE/mL).
  5. eData of 10 pigs, which tested positive.
  6. fData of 2 pigs, which tested positive.
  7. gPiglets of control 1 group were housed in the same room as G5 piglets.