Table 1 List of genes and sequences of the primers used for qPCR analysis.
Gene name | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | Accession number |
|---|---|---|---|
β-actin | CAGGTCATCACCATCGGCAACG | GACAGCACCGTGTTGGCGTAGAGGT | U07786 |
PPIA | CCTGAACATACGGGTCCTG | AACTGGGAACCGTTTGTGTTG | AY266299 |
18S | GACTCAACACGGGAAACCTCAC | GCTTATGACCCGCACTTACTGG | AY265350 |
TNFα | CCTCTTCTCCTTCCTCCTG | CCTCGGCTTTGACATTGG | X57321 |
IL-1β | GGCCGCCAAGATATAACTGA | GGACCTCTGGGTATGGCTTTC | NM_214055 |
IL-4 | TTGCTGCCCCAGAGAAC | TGTCAAGTCCGCTCAGG | AY294020 |
IL-5 | TGGTGGCAGAGACCTTGACA | CCATCGCCTATCAGCAGAGTT | NM_214205.1 |
IL-6 | TGGCTACTGCCTTCCCTACC | CAGAGATTTTGCCGAGGATG | NM_214399 |
IL-8 | TTCGATGCCAGTGCATAAATA | CTGTACAACCTTCTGCACCCA | AB057440 |
IL-10 | CAGATGGGCGACTTGTTG | ACAGGGCAGAAATTGATGAC | L20001 |
IL-12p40 | GGAGTATAAGAAGTACAGAGTGG | GATGTCCCTGATGAAGAAGC | U08317 |
IL-13 | AAGTGGCCCAGTTCGTAAAAGA | ACCCGTGGCGAAAAATCA | NM_213803.1 |
IL-17A | CTCTCGTGAAGGCGGGAAT | TCCTCAGTTTTTGGGCATCCT | NM_001005729 |
IL-18 | AGGGACATCAAGCCGTGTTT | CGGTCTGAGGTGCATTATCTGA | EU118362.1 |
IL-21 | CTGCCTGATGGTCATCTTCTCA | AGGCGATCTTGTCCTTGGAA | NM_214415 |
IL-23 | GCTTGCAAAGGATCCACCAA | GGCTCCCCTGTGAAAATGTC | NM_001130236.1 |
IFN-γ | CAAAGCCATCAGTGAACTCATCA | TCTCTGGCCTTGGAACATAGTCT | X53085 |
TGF-βR1 | TCAGGCTTACCATTGCTTGTTCAG | CTCGCCAAACCTCTCCAAATCG | NM_001038639 |
DEFB1 | ACCGCCTCCTCCTTGTATTC | GGTGCCGATCTGTTTCATCT | NM_213838 |
DEFB2 | CTGTCTGCCTCCTCTCTTCC | CAGGTCCCTTCAATCCTGTT | NM_214442 |
NF-κB | CTCGCACAAGGAGACATGAA | ACTCAGCCGGAAGGCATTAT | DQ834921 |
CD163 | GCCTGTCTCATCGCATTCCT | GGATTTAGCATATCCGTTTCATCTG | NM_213976.1 |