Live attenuated African swine fever viruses as ideal tools to dissect the mechanisms involved in viral pathogenesis and immune protection

Table 2 Histopathology evaluation (experiment 2). Quantification of macrophage infiltrations (A), presence of apoptotic cells (B) and ASFV antigen detection (C) in gastrohepatic lymph node.

	Animal	Day 1 pi	Day 3 pi	Day 7 pi	Day 31 pi
(A)
Macrophage infiltration (MAC387⁺)
E75	1	+	+	+++
	2	+	+	+++
	3	+	+	++
E75CV1	1	+	+	+	+
	2	++	++	++	+
	3	++	+	++	+
Control	1	+	+	+	+
	2	+	+	+	+
	3	+	+	+	+
(B)
Apoptotic cell quantification (Caspase-3⁺)
E75	1	+	+	+++
	2	+	+	++
	3	+	+	++
E75CV1	1	+	+	+	+
	2	+	++	+	+
	3	+	+	+	+
Control	1	+	+	+	+
	2	+	+	+	+
	3	+	+	+	+
(C)
ASFV Antigen detection (p30⁺)
E75	1	+	++	+++
	2	–	+	++
	3	–	++	+++
E75CV1	1	+	–	+	+
	2	–	–	+	+
	3	+	+	–	++

Results were quantified as follows: (–) no detection, (+) low, (++) moderate and (+++) high amounts

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