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Table 1 Experimental infections of P. perniciosus with L. infantum parasites.

From: Natural transmission of Leishmania infantum through experimentally infected Phlebotomus perniciosus highlights the virulence of Leishmania parasites circulating in the human visceral leishmaniasis outbreak in Madrid, Spain

Nº experiment

Leishmania strain or isolate

Infective mixture (parasites/mL)

Fed flies (%)a

Flies transfered to pots (%)b

Surviving flies after oviposition (%)c

Positive flies (%)d

1

JPCM5

2.5 × 108

395/500 (79.0)

332/395 (84.1)

220/332 (66.3)

72/100 (72.0)

2

JPCM5

2.5 × 108

425/500 (85.0)

216/425 (50.8)

196/216 (90.7)

88/96 (91.7)

3

POL2FL6

2.5 × 107

317/500 (63.4)

240/317 (75.7)

150/240 (62.5)

18/21 (85.7)

4

POL2FL6

2.5 × 107

303/500 (60.6)

151/303 (49.8)

17/151 (11.3)

15/17 (88.3)

  1. aBlood-fed sand flies/exposed sand flies ×100
  2. bFlies transferred to pots/blood-fed sand flies ×100
  3. cFlies that survived oviposition/flies transferred to pots ×100
  4. d Leishmania positive sand flies/dissected sand flies ×100