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Table 3 Impact of the fourteen highly conserved residues on activity of HSV-1 vhs protein and other nucleases.

From: Roles of nucleic acid substrates and cofactors in the vhs protein activity of pseudorabies virus

Residues corresponding to PrV vhs

Corresponding residues

HSV vhs

T4 RNase H

Human FEN-1

 

RNase activity [13]

vhs activity [24]

eIF4H binding [13]

 

Mg2+ binding [31, 32]

Nuclease /substrate binding [23, 35, 36]

 

Mg2+ binding [32, 34, 37]

Nuclease /substrate binding [33]

D34

D34

+

D19

D34

D81

D82

+

D71

D86

K95

K96

N

K87

N

R103

N

+

E150

E192

+

E130

N

N

E158

D152

D194

+

D132

E160

N

D169

T211

<

S153

<

T177

N

N

D171

D213

+

D155

D179

+

D172

T214

D156

N

N

D180

N

N

D173

D215

+

D157

D181

D219

D261

<

+

D200

+

D233

P343

P465

N

N

N

N

E357

N

N

P345

P467

N

N

N

N

E359

N

N

L352

L474

N

N

N

N

K366

N

N

W356

W478

N

N

N

N

G370

N

N

  1. “−” indicates the point mutations lost RNase activity and the ability of Mg2+ or eIF4H binding.
  2. “<” indicates the point mutations partially reduced RNase activity and the ability of Mg2+ binding.
  3. “N” indicates the impact of those residues have not yet been defined.
  4. indicates FEN-1 D181 mutation affects their ability of ribonucleases, but does not affect its ability to bind with the substrate.